The AAO Journal, 2024/06, 34(2):33. doi: 10.53702/i2375-5717-34.2.16, type of study: ,ani,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,

Full text    (https://meridian.allenpress.com/aaoj/article/34/2/16/500954/LBORC-NUFA-Poster-Abstracts-2024-Students)

Keywords:

animal experiment [68]
blood glucose [5]
insulin sensitivity [1]
OMT [3924]
osteopathic manipulative treatment [3945]

Abstract:

Introduction/Background: Blood glucose (BG) homeostasis is maintained via the autonomic nervous system, thoracolumbar diaphragm, abdominal lymphatic system, pancreas, liver, and adrenal glands. Our proof-of-concept study demonstrated the feasibility of performing osteopathic manipulative treatment (OMT) on adult rats. We hypothesized that osteopathic manipulation to the above areas in rodents will affect BG levels. Based on pilot data we also hypothesized that using isoflurane anesthetic affects blood glucose. Methods: Two groups of 9 male rats were fed either a normal chow diet or a high-fat diet (HFD) to induce obesity and insulin resistance. Their weight and BG levels were monitored biweekly. The rats were anesthetized using isoflurane. The treatment group received OMT (rib raising, diaphragmatic doming, and prevertebral ganglia, suboccipital, and thoracic outlet release). Sham group received a light touch. Control group received no treatment. BG was measured using a glucometer on blood from the tail pre-treatment and 5 and 15 minutes post-treatment. Paired t-testing was utilized to determine a significant difference in BG. Results: Among HFD rats, BG levels increased at 5 and 15 minutes post-treatment. The percent change in BG from pre- to 15 minutes post-treatment was significantly higher than that from pre- to 5 minutes post-treatment (p<0.001, T=-4.50). Conclusions and Discussion: We found that blood glucose significantly increases in HFD rats with more time post-treatment. We recognize isoflurane may have a confounding effect, as time post-treatment directly correlates to isoflurane exposure. A larger population is needed to assess both acute and long-term effects of OMT on insulin sensitivity and BG homeostasis. Study limitations included rodent sex, age, weight, anesthesia usage, and sample size. Future studies will use both males and females to account for size and temperament differences accompanying sex.