The Journal of the American Osteopathic Association, 2009/08, 109(8):454. doi: 10.7556/jaoa.2009.109.8.425, type of study: ,ani,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,

Full text    (https://www.degruyter.com/document/doi/10.7556/jaoa.2009.109.8.425/html)

Keywords:

animal experiment [68]
LPT [27]
lymphatic pump technique [43]
lymphatic system [58]

Abstract:

Hypothesis: Lymph stasis can result in edema and accumulation of particulate matter, exudates, toxins, and bacteria. This can lead to inflammation, impaired immune trafficking, tissue hypoxia, tissue fibrosis, and a variety of diseases. In earlier studies, using a canine model, we demonstrated that lymphatic pump treatment (LPT) significantly increased thoracic and intestinal duct lymph flow and leukocyte concentrations. It is likely that this increase in lymphatic flow during LPT facilitates the release of inflammatory mediators from tissues into circulation. Methods: To determine the acute effects of LPT on lymphatic cytokine and chemokine concentrations, a catheter was inserted into either the thoracic (n=6) or intestinal (n=6) lymph ducts of mongrel dogs. Lymph was collected during 4 minutes of resting (baseline), during 4 minutes of LPT, and during 10 minutes following LPT. The concentrations of IL-4, IL-6, IL-8, IL-10, IL-15, MCP-1, TNF-a, INF-y, KC (CXCL1), and MCP-1 were measured in both thoracic and intestinal duct lymph using a multiplex assay. Results: On average, LPT increased thoracic duct lymph cytokine/chemokine concentrations approximately tenfold, and intestinal lymph cytokines/chemokine concentrations approximately fivefold compared to baseline. Furthermore, by 10 minutes following cessation of LPT (recovery), thoracic and intestinal lymph cytokine/chemokine concentrations were similar to baseline, suggesting their release is transient. There was no preferential release of either pro- or anti-inflammatory cytokines/chemokines during LPT; however, the greatest increases were seen in IL-2, IL-6, IL-8, IL-10, KC, and MCP-1. Conclusion: The results from this study demonstrate that, in addition to enhancing lymph flow and lymphatic leukocyte concentrations, LPT is able to mobilize inflammatory mediators into lymphatic circulation. This redistribution of inflammatory mediators during LPT may provide scientific rationale for the clinical use of LPT to enhance immunity and treat infection.