Advanced search

Search results      


Osteopathy In The Cranial Field As A Method To Enhance Injury Recovery: A Preliminary Study

Journal: Journal of Osteopathic Medicine Date: 2019/12, 119(12):Pages: e78-e79. doi: Subito , type of study: animal experiment

Full text    (https://www.degruyter.com/document/doi/10.7556/jaoa.2019.128/html)

Keywords:

animal experiment [36]
brain injury [20]
cranio-sacral osteopathy [158]
injuries [66]
LPT [3]
lymphatic pump technique [21]
pilot study [104]
recovery [14]
trauma [100]

Abstract:

Statement of Significance: TBI represents a significant clinical burden with over 2 million cases each year. Patients that sustain mild TBI are at greater risk of developing neurodegenerative diseases, such as Alzheimer disease, in addition to experiencing cognitive and psychological deficits. Currently, no therapeutic strategies have proven successful in mitigating the effects of TBI, thus novel strategies must be considered to improve the quality of life of TBI patients. Evidence on the clinical efficacy of OCF is heterogeneous and insufficient to draw definitive conclusions. Studies have found that the quality of life was improved when OCF was used for children with cerebral palsy. Others reported positive changes in pain and autonomic nervous system function. Methods: Male adult rats were used to determine blood-brain-barrier permeability following OCF. All animal procedures were approved prior to conducting in accordance with VT IACUC protocols. Anesthetized rats were treated with OCF therapy or control (sham). A board certified osteopathic physician with additional training in OCF performed OCF therapy on animals. Light pressure was held until the operator felt the tissue relax, which lead to enhanced lymphatic drainage. Next, the Lymphatic Pump Technique (LPT) was performed. The operator pressed the abdomen of the anesthetized rat with the thumb on one side and the index and middle fingers on the other side of the medial sagittal plane. The fingers were placed bilaterally, caudal to the ribs. Previous studies showed that similar procedures of LPT were successful [1-6]. Next Evans Blue (EB) solution was injected intravenously. After 24 hours, animals were transcardially perfused with saline, and brains were collected, weighed and homogenized. Supernatants of each tissue were measured. A second round of testing was conducted to determine if OCF influences CSF flow within the brain. Tracers were delivered into the cisterna magna of anesthetized rats and conjugated dextran and ovalbumin were administered. Ten minutes after infusion, rats underwent OCF and LPT. One hour following therapy, rats were transcardially perfused and fixed with 4% paraformaldehyde. Brains were collected, sectioned and tracer levels were measured. Data Analysis: EB dye concentration was calculated as the ratio of absorbance relative to the amount of tissue staining into the brain. Data were analyzed using 1-way ANOVA with Tukey post hoc t tests for pairs of data (OCF verses sham). Quantification of the fluorescent CSF tracers flow into the brain was conducted using NIH Image J software. Drainage was determined by measuring the distance of tracers into the brain. The fluorescence area coverage from the brain slices from each animal was averaged to define CSF penetration within a single biological replicate. Results: For the EB study, we found no significant difference (P=.89) between the brain homogenates, indicating that OCF did not cause BBB disruption in naïve animals. Brains have been collected from the tracer study animals and are in the process of begin analyzed. Conclusion: One possible explanation for the health benefit provided by OCF would be that the manipulation enhances the flow of CSF within the brain. This innovative research allows for tracking particles and the quantification of flow motion within the brain to begin modeling the physiological response to the OCF treatment. The basic knowledge gained will build a foundation for designing effective noninvasive rehabilitation therapies for TBI patients.


Search results      

 
 
 






  • ImpressumLegal noticeDatenschutz


ostlib.de/data_zkvmxtefpuhwaqcbysdg



Supported by

OSTLIB recommends